| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1531 | Brevibacillus brevis | TCACGATGCTGATCCTGGC | AGCTGGTTGCAATCGCCA | 59.86 | 60.28 | 216 | 65 |
100.00%
|
100.00%
|
| Primer 1532 | Brevibacillus brevis | GACCTTGCAAACTGCTCGC | AGAGTGATGTCGGACGGGA | 60.08 | 60.00 | 183 | 65 |
100.00%
|
100.00%
|
| Primer 1533 | Brevibacillus brevis | ACCTTGCAAACTGCTCGC | ATGTCGGACGGGATTGCTC | 58.97 | 59.86 | 176 | 65 |
100.00%
|
100.00%
|
| Primer 1534 | Brevibacillus brevis | ACCTTGCAAACTGCTCGC | GATGTCGGACGGGATTGCT | 58.97 | 59.86 | 177 | 65 |
100.00%
|
100.00%
|
| Primer 1535 | Brevibacillus brevis | ACCTTGCAAACTGCTCGC | TGATGTCGGACGGGATTGC | 58.97 | 60.15 | 178 | 65 |
100.00%
|
100.00%
|
| Primer 1536 | Brevibacillus brevis | CACCGAATCCGAACACCCA | TTTGGCAGAACGGCTCCA | 60.00 | 59.49 | 233 | 65 |
100.00%
|
100.00%
|
| Primer 1537 | Brevibacillus brevis | CCAGAGTGGGCAACAAAATGG | CCCTGCGAGAACAGTTACCA | 60.00 | 59.68 | 189 | 65 |
100.00%
|
100.00%
|
| Primer 1538 | Brevibacillus brevis | TGTACCCGTTTCGCGACA | TTGCACCTGACAACGCCA | 59.27 | 60.12 | 179 | 65 |
100.00%
|
100.00%
|
| Primer 1539 | Brevibacillus brevis | GCGACTTTGGCTGCATGT | GTCCCTTCATCGTCGGCTT | 59.04 | 59.78 | 161 | 65 |
100.00%
|
100.00%
|
| Primer 1540 | Brevibacillus brevis | AACCGATGCAGCTGACCA | ATGCTCAACGGCACCGAT | 59.25 | 59.73 | 200 | 65 |
100.00%
|
100.00%
|